mouse SMPD3 shRNA silencing Adenovirus

Name 
Ad-m-SMPD3-shRNA
Cat No 
shADV-272660
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Smpd3.

shADV-272660
Ad-m-SMPD3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Smpd3 (sphingomyelin phosphodiesterase 3, neutral). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
4631433G07Rik; AI427456; AW537966; fro; nSMase2
Description (Vector)
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Gene ID
58994
Gene Name (Vector)
sphingomyelin phosphodiesterase 3, neutral
Gene Symbol
Smpd3
ORF Size (aa)
1968
ORF Size (bp)
1968 bp
RefSeq ID
NM_021491
RefSeq Synonyms
NM_021491, BC046980, BC043077,
Species
mouse
UniGene ID
Mm.23298

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.