mouse SETD8 shRNA silencing Adenovirus

Name 
Ad-m-SETD8-shRNA
Cat No 
shADV-271763
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Kmt5a.

shADV-271763
Ad-m-SETD8-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Kmt5a (lysine methyltransferase 5A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2410195B05Rik; AA617402; AW536475; PR-SET7; PR/SET07; Setd8
Description (Vector)
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.
Gene ID
67956
Gene Name (Vector)
lysine methyltransferase 5A
Gene Symbol
Kmt5a
ORF Size (aa)
1050
ORF Size (bp)
1050 bp
RefSeq ID
NM_030241
RefSeq Synonyms
NM_030241, NM_001310727, NM_001310725, NM_001310723, BC108333,
Species
mouse
UniGene ID
Mm.137966

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.