mouse RRP8 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Rrp8.
shADV-271160
Ad-m-RRP8-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rrp8 (ribosomal RNA processing 8, methyltransferase, homolog (yeast)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
1500003O22Rik; 2900001K19Rik; AW538116
Description (Vector)
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Gene ID
101867
Gene Name (Vector)
ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Gene Symbol
Rrp8
ORF Size (aa)
1374
ORF Size (bp)
1374 bp
RefSeq ID
NM_025897
RefSeq Synonyms
NM_133951, NM_025897, BC046799, BC022923,
Species
mouse
UniGene ID
Mm.231400
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.