mouse RPA3 shRNA silencing Adenovirus

Name 
Ad-m-RPA3-shRNA
Cat No 
shADV-270822
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Rpa3.

shADV-270822
Ad-m-RPA3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rpa3 (replication protein A3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
14kDa; C330026P08Rik
Description (Vector)
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions|Function: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Gene ID
68240
Gene Name (Vector)
replication protein A3
Gene Symbol
Rpa3
ORF Size (aa)
366
RefSeq ID
NM_026632
RefSeq Synonyms
NM_026632, BC028489,
Species
mouse
UniGene ID
Mm.29073

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.