mouse PIAS2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Pias2.
shADV-268597
Ad-m-PIAS2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Pias2 (protein inhibitor of activated STAT 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
6330408K17Rik; AI462206; ARIP3; AU018068; Dib; Miz1; PIASxalpha; PIASxb; PIASxbeta; SIZ2
Description (Vector)
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
Gene ID
17344
Gene Name (Vector)
protein inhibitor of activated STAT 2
Gene Symbol
Pias2
ORF Size (aa)
1839
ORF Size (bp)
1839 bp
RefSeq ID
NM_008602
RefSeq Synonyms
NM_008602, NM_001164170, NM_001164169, NM_001164168, NM_001164167, BC034711, BC005596,
Species
mouse
UniGene ID
Mm.6370
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.