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mouse MTPAP shRNA silencing Adenovirus

Name 
Ad-m-MTPAP-shRNA
Cat No 
shADV-265165
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Mtpap.

shADV-265165
Ad-m-MTPAP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mtpap (mitochondrial poly(A) polymerase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
0610027A18Rik; AW551379; Papd1
Description (Vector)
Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
Gene ID
67440
Gene Name (Vector)
mitochondrial poly(A) polymerase
Gene Symbol
Mtpap
ORF Size (aa)
1758
ORF Size (bp)
1758 bp
RefSeq ID
NM_026157
RefSeq Synonyms
NM_026157, BC057643,
Species
mouse
UniGene ID
Mm.49826

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.