mouse MIF shRNA silencing Adenovirus

Name 
Ad-m-MIF-shRNA
Cat No 
shADV-264643
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Mif.

shADV-264643
Ad-m-MIF-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mif (macrophage migration inhibitory factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
93
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
GIF; Glif
Description (Vector)
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Gene ID
17319
Gene Name (Vector)
macrophage migration inhibitory factor
Gene Symbol
Mif
ORF Size (aa)
348
RefSeq ID
NM_010798
RefSeq Synonyms
NM_010798, BC100605, BC086928, BC024895,
Species
mouse
UniGene ID
Mm.2326

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.