mouse MCF2 shRNA silencing Adenovirus

Name 
Ad-m-MCF2-shRNA
Cat No 
shADV-264361
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Mcf2.

shADV-264361
Ad-m-MCF2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mcf2 (mcf.2 transforming sequence). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
B230117G22Rik; Dbl; Mcf-2
Description (Vector)
Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Gene ID
109904
Gene Name (Vector)
mcf.2 transforming sequence
Gene Symbol
Mcf2
ORF Size (aa)
2787
ORF Size (bp)
2787 bp
RefSeq ID
NM_133197
RefSeq Synonyms
NM_133197, NM_001289731, NM_001289730, BC125435,
Species
mouse
UniGene ID
Mm.93796

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.