mouse MAPKAP1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Mapkap1.
shADV-264233
Ad-m-MAPKAP1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mapkap1 (mitogen-activated protein kinase associated protein 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI591529; D230039K05Rik; Sin1
Description (Vector)
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
Gene ID
227743
Gene Name (Vector)
mitogen-activated protein kinase associated protein 1
Gene Symbol
Mapkap1
ORF Size (aa)
1569
ORF Size (bp)
1569 bp
RefSeq ID
NM_177345
RefSeq Synonyms
NM_177345, NM_001290626, NM_001290625, BC096618, BC090644,
Species
mouse
UniGene ID
Mm.270866
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.