mouse LPCAT2 shRNA silencing Adenovirus

Name 
Ad-m-LPCAT2-shRNA
Cat No 
shADV-263773
Availability
5-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Lpcat2.

shADV-263773
Ad-m-LPCAT2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Lpcat2 (lysophosphatidylcholine acyltransferase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
93
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A330042H22; Aytl1; Aytl1a; lpafat1
Description (Vector)
Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (By similarity).
Gene ID
270084
Gene Name (Vector)
lysophosphatidylcholine acyltransferase 2
Gene Symbol
Lpcat2
ORF Size (aa)
1635
ORF Size (bp)
1635 bp
RefSeq ID
NM_173014
RefSeq Synonyms
NM_173014, BC160287, BC150666, BC144806,
Species
mouse
UniGene ID
Mm.21463

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.