mouse UBE2I shRNA silencing Adenovirus

Name 
Ad-m-UBE2I-shRNA
Cat No 
shADV-263625
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Ube2i.

shADV-263625
Ad-m-UBE2I-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ube2i (ubiquitin-conjugating enzyme E2I). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
5830467E05Rik; F830028O17Rik; UBC9; Ubce2i; Ubce9
Description (Vector)
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
Gene ID
22196
Gene Name (Vector)
ubiquitin-conjugating enzyme E2I
Gene Symbol
Ube2i
ORF Size (aa)
306
ORF Size (bp)
306 bp
RefSeq ID
NM_011665
RefSeq Synonyms
NM_011665, NM_001177610, NM_001177609, BC037635,
Species
mouse
UniGene ID
Mm.240044

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.