mouse GZMB shRNA silencing Adenovirus

Name 
Ad-m-GZMB-shRNA
Cat No 
shADV-260975
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Gzmb.

shADV-260975
Ad-m-GZMB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Gzmb (granzyme B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AI553453; CCP1; CCP-1/C11; Ctla1; Ctla-1; cytotoxic cell protease 1; fragmentin-2; granzyme B(G,H); GZB
Description (eCommerce)
Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing [taken from NCBI Entrez Gene (Gene ID: 3002)]
Description (Vector)
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Gene ID
14939
Gene Name (eCommerce)
Granzyme B
Gene Name (Vector)
granzyme B
Gene Symbol
Gzmb
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
744
Protein Name (eCommerce)
Granzyme B(G,H)
RefSeq ID
NM_013542
RefSeq Synonyms
NM_013542, BC002085,
Research Areas
Apoptosis,Cancer,Immune Cells & Tumor Markers,Immunology,Immuno-Oncology,Interleukin Signaling,Neurobiology,Notch Signaling,Signal Transduction,immunology,cell_biology,T-cell Receptor Signaling,Toll-like Receptor Signaling,Transcriptional Misregulation in Cancer
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,immuno_oncology,immunology,neurobiology,signal_transduction,transcription_translation
Species
mouse
Target Sentence
Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing [taken from NCBI Entrez Gene (Gene ID: 3002)]
UniGene ID
Mm.14874
UniProt ID (eCommerce)
P04187

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.