mouse EPAS1 shRNA silencing Adenovirus

Name 
Ad-m-EPAS1-shRNA
Cat No 
shADV-258542
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Epas1.

shADV-258542
Ad-m-EPAS1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Epas1 (endothelial PAS domain protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
78
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2
Description (Vector)
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Gene ID
13819
Gene Name (Vector)
endothelial PAS domain protein 1
Gene Symbol
Epas1
ORF Size (aa)
2625
ORF Size (bp)
2625 bp
RefSeq ID
NM_010137
RefSeq Synonyms
NM_010137, BC057870,
Species
mouse
UniGene ID
Mm.1415

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.