mouse DICER1 shRNA silencing Adenovirus

Name 
Ad-m-DICER1-shRNA
Cat No 
shADV-257176
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Dicer1.

shADV-257176
Ad-m-DICER1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Dicer1 (dicer 1, ribonuclease type III). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110006F08Rik; D12Ertd7e; mKIAA0928
Description (Vector)
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Gene ID
192119
Gene Name (Vector)
dicer 1, ribonuclease type III
Gene Symbol
Dicer1
ORF Size (aa)
5718
ORF Size (bp)
5718 bp
RefSeq ID
NM_148948
RefSeq Synonyms
NM_148948, BC172621,
Species
mouse
UniGene ID
Mm.21135

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.