mouse DHFR shRNA silencing Adenovirus

Name 
Ad-m-DHFR-shRNA
Cat No 
shADV-257129
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Dhfr.

shADV-257129
Ad-m-DHFR-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Dhfr (dihydrofolate reductase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
8430436I03Rik; AA607882; AI662710; AW555094
Description (Vector)
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Gene ID
13361
Gene Name (Vector)
dihydrofolate reductase
Gene Symbol
Dhfr
ORF Size (aa)
564
RefSeq ID
NM_010049
RefSeq Synonyms
NM_010049, BC005796,
Species
mouse
UniGene ID
Mm.23695

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.