mouse D10WSU52E shRNA silencing Adenovirus

Name 
Ad-m-D10WSU52E-shRNA
Cat No 
shADV-256617
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Rtcb.

shADV-256617
Ad-m-D10WSU52E-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rtcb (RNA 2′,3′-cyclic phosphate and 5′-OH ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AI255213; AI463255; D10Wsu52e; FAAP; HSPC117
Description (Vector)
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as a RNA ligase with broad substrate specificity, and may function toward other RNAs.
Gene ID
28088
Gene Name (Vector)
RNA 2',3'-cyclic phosphate and 5'-OH ligase
Gene Symbol
Rtcb
ORF Size (aa)
1518
ORF Size (bp)
1518 bp
RefSeq ID
NM_145422
RefSeq Synonyms
NM_145422, BC003288,
Species
mouse
UniGene ID
Mm.9257

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.