mouse CUL4A shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Cul4a.
shADV-256372
Ad-m-CUL4A-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Cul4a (cullin 4A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2810470J21Rik; AW495282
Description (Vector)
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
Gene ID
99375
Gene Name (Vector)
cullin 4A
Gene Symbol
Cul4a
ORF Size (aa)
1785
ORF Size (bp)
1785 bp
RefSeq ID
NM_146207
RefSeq Synonyms
NM_146207, BC024113, BC010211,
Species
mouse
UniGene ID
Mm.212861
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.