mouse CRTC2 shRNA silencing Adenovirus

Name 
Ad-m-CRTC2-shRNA
Cat No 
shADV-256154
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Crtc2.

shADV-256154
Ad-m-CRTC2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Crtc2 (CREB regulated transcription coactivator 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
4632407F12Rik; mTORC2; Torc2
Description (Vector)
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Gene ID
74343
Gene Name (Vector)
CREB regulated transcription coactivator 2
Gene Symbol
Crtc2
ORF Size (aa)
2079
ORF Size (bp)
2079 bp
RefSeq ID
NM_028881
RefSeq Synonyms
NM_028881, BC033300, BC032183, BC023831, BC023091,
Species
mouse
UniGene ID
Mm.35627

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.