mouse CHST12 shRNA silencing Adenovirus

Name 
Ad-m-CHST12-shRNA
Cat No 
shADV-255474
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Chst12.

shADV-255474
Ad-m-CHST12-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Chst12 (carbohydrate sulfotransferase 12). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AI595374; C4S-2; C4ST2
Description (Vector)
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Gene ID
59031
Gene Name (Vector)
carbohydrate sulfotransferase 12
Gene Symbol
Chst12
ORF Size (aa)
1260
ORF Size (bp)
1260 bp
RefSeq ID
NM_021528
RefSeq Synonyms
NM_021528, BC003201,
Species
mouse
UniGene ID
Mm.28934

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.