mouse BCKDK shRNA silencing Adenovirus

Name 
Ad-m-BCKDK-shRNA
Cat No 
shADV-253872
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Bckdk.

shADV-253872
Ad-m-BCKDK-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Bckdk (branched chain ketoacid dehydrogenase kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AI327402
Description (Vector)
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).
Gene ID
12041
Gene Name (Vector)
branched chain ketoacid dehydrogenase kinase
Gene Symbol
Bckdk
ORF Size (aa)
1239
ORF Size (bp)
1239 bp
RefSeq ID
NM_009739
RefSeq Synonyms
NM_009739, BC046595,
Species
mouse
UniGene ID
Mm.425495

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.