mouse BCKDHB shRNA silencing Adenovirus

Name 
Ad-m-BCKDHB-shRNA
Cat No 
shADV-253871
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Bckdhb.

shADV-253871
Ad-m-BCKDHB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Bckdhb (branched chain ketoacid dehydrogenase E1, beta polypeptide). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BCKDE1B; BCKDH E1-beta
Description (Vector)
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Gene ID
12040
Gene Name (Vector)
branched chain ketoacid dehydrogenase E1, beta polypeptide
Gene Symbol
Bckdhb
ORF Size (aa)
969
RefSeq ID
NM_199195
RefSeq Synonyms
NM_199195, NM_001305935, BC064099,
Species
mouse
UniGene ID
Mm.12819

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.