mouse ATP5J shRNA silencing Adenovirus

Name 
Ad-m-ATP5J-shRNA
Cat No 
shADV-253307
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Atp5j.

shADV-253307
Ad-m-ATP5J-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Atp5j (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CF6
Description (Vector)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
Gene ID
11957
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F
Gene Symbol
Atp5j
ORF Size (aa)
327
RefSeq ID
NM_016755
RefSeq Synonyms
NM_016755, NM_001302216, NM_001302215, NM_001302214, NM_001302213, BC010766,
Species
mouse
UniGene ID
Mm.353

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.