mouse ATP5E shRNA silencing Adenovirus

Name
Ad-m-ATP5E-shRNA

Cat No
shADV-253296

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Atp5e.

shADV-253296

Ad-m-ATP5E-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Atp5e (ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

2410043G19Rik; ATPE; AV000645

Description (Vector)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).

Gene ID

67126

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit

Gene Symbol

Atp5e

ORF Size (aa)

159

RefSeq ID

NM_025983

RefSeq Synonyms

NM_025983, BC024339,

Species

mouse

UniGene ID

Mm.20841

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.