mouse ATG4D shRNA silencing Adenovirus

Name 
Ad-m-ATG4D-shRNA
Cat No 
shADV-253224
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Atg4d.

shADV-253224
Ad-m-ATG4D-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Atg4d (autophagy related 4D, cysteine peptidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
9830134P12Rik; APG4-D; Apg4d; Atg4dl; Autl4
Description (Vector)
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Gene ID
235040
Gene Name (Vector)
autophagy related 4D, cysteine peptidase
Gene Symbol
Atg4d
ORF Size (aa)
1425
ORF Size (bp)
1425 bp
RefSeq ID
NM_153583
RefSeq Synonyms
NM_153583, BC069851, BC030861,
Species
mouse
UniGene ID
Mm.440165

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.