mouse BRF1 shRNA silencing Adenovirus

Name 
Ad-m-BRF1-shRNA
Cat No 
shADV-251538
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Brf1.

shADV-251538
Ad-m-BRF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Brf1 (BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2510002F24Rik; GTF3B; mTFIIIB90; TAF3C; TAFIII90; TFIIIB90
Description (Vector)
General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Gene ID
72308
Gene Name (Vector)
BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Gene Symbol
Brf1
ORF Size (aa)
2031
ORF Size (bp)
2031 bp
RefSeq ID
NM_028193
RefSeq Synonyms
NM_028193, BC037224,
Species
mouse
UniGene ID
Mm.196721

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.