human SIRPA shRNA silencing Adenovirus

Name 
Ad-h-SIRPA-shRNA
Cat No 
shADV-231063
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SIRPA.

shADV-231063
Ad-h-SIRPA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SIRPA (signal regulatory protein alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BIT; Brain Ig-like molecule with tyrosine-based activation motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD antigen CD172a; CD172 antigen-like family member A; CD172A; inhibitory receptor SHPS-1; macrophage fusion receptor; MFR; MYD-1; myd-1 antigen; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1
Description (eCommerce)
SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha is a cell surface receptor for CD47 and CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [taken from NCBI Entrez Gene (Gene ID: 140885)].
Description (Vector)
SIRPA is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
Gene ID
140885
Gene Name (eCommerce)
signal regulatory protein alpha
Gene Name (Vector)
signal regulatory protein alpha
Gene Symbol
SIRPA
HGNC ID
HGNC:9662
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1527
ORF Size (bp)
1527 bp
Protein Name (eCommerce)
Tyrosine-protein phosphatase non-receptor type substrate 1
RefSeq ID
NM_080792
RefSeq Synonyms
NM_080792, NM_001330728, NM_001040023, NM_001040022, BC075849, BC038510, BC033092, BC026692,
Research Areas
BDNF Signaling,Cardiac Development,Cardiology,Cardiovascular,EGFR Signaling,Immunology,Neurobiology,Prolactin Signaling,Signal Transduction
Research Areas (Faceted)
cardiovascular,developmental_biology,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha is a cell surface receptor for CD47 and CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [taken from NCBI Entrez Gene (Gene ID: 140885)].
UniGene ID
Hs.581021
UniProt ID (eCommerce)
P78324

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.