human RBM17 shRNA silencing Adenovirus

Name 
Ad-h-RBM17-shRNA
Cat No 
shADV-230173
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RBM17.

shADV-230173
Ad-h-RBM17-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RBM17 (RNA binding motif protein 17). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
45 kDa-splicing factor; RNA-binding motif protein 17; SPF45; splicing factor 45; splicing factor 45kDa
Description (eCommerce)
Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96125].
Description (Vector)
RBM17 encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15.
Gene ID
84991
Gene Name (eCommerce)
RNA binding motif protein 17
Gene Name (Vector)
RNA binding motif protein 17
Gene Symbol
RBM17
HGNC ID
HGNC:16944
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1206
ORF Size (bp)
1206 bp
Protein Name (eCommerce)
Splicing factor 45
RefSeq ID
NM_032905
RefSeq Synonyms
NM_032905, NM_001145547, BC039322, BC007871,
Research Areas
Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96125].
UniGene ID
Hs.498548
UniProt ID (eCommerce)
Q96I25

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.