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human ZAK shRNA silencing Adenovirus

Name 
Ad-h-ZAK-shRNA
Cat No 
shADV-228028
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ZAK.

shADV-228028
Ad-h-ZAK-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ZAK (sterile alpha motif and leucine zipper containing kinase AZK). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AZK; CNM6; HCCS-4; mitogen-activated protein kinase kinase kinase 20; mitogen-activated protein kinase kinase kinase MLT; mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; MLK7; mlklak; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; SFMMP; sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; ZAK; ZAK1 homolog, leucine zipper and sterile-alpha motif kinase
Description (eCommerce)
Leucine-zipper and sterile-alpha motif kinase (ZAK) is a mitogen activated protein kinase kinase kinase involved in the S and G2 phase cell cycle checkpoint. ZAK has been shown to activate the stress-activated protein kinase (c-jun N-terminal kinase) pathway and NF-kappaB. It has also been found to be a key mediator of cardiomyocyte hypertrophy and myocardial scars.
Description (Vector)
ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID
51776
Gene Name (eCommerce)
mitogen-activated protein kinase kinase kinase 20
Gene Name (Vector)
sterile alpha motif and leucine zipper containing kinase AZK
Gene Symbol
ZAK
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1368
ORF Size (bp)
1368 bp
Protein Name (eCommerce)
Mitogen-activated protein kinase kinase kinase MLT
RefSeq ID
NM_016653
RefSeq Synonyms
NM_133646, NM_016653, BC001401,
Research Areas
Apoptosis,Cell Biology,Cell Cycle,Cytoskeleton,DNA Damage/Repair,Kinase/Phosphatase,MAPK Signaling,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,cell_biology,signal_transduction
Species
human
Target Sentence
Leucine-zipper and sterile-alpha motif kinase (ZAK) is a mitogen activated protein kinase kinase kinase involved in the S and G2 phase cell cycle checkpoint. ZAK has been shown to activate the stress-activated protein kinase (c-jun N-terminal kinase) pathway and NF-kappaB. It has also been found to be a key mediator of cardiomyocyte hypertrophy and myocardial scars.
UniGene ID
Hs.444451
UniProt ID (eCommerce)
Q9NYL2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.