Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human YAF2 shRNA silencing Adenovirus

Name 
Ad-h-YAF2-shRNA
Cat No 
shADV-227944
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human YAF2.

shADV-227944
Ad-h-YAF2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human YAF2 (YY1 associated factor 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
YY1-associated factor 2
Description (eCommerce)
YY1-associated factor 2 (YAF2) binds to MYC and inhibits MYC-mediated transactivation. YAF2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. YAF2 is a component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8IY57].
Description (Vector)
YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple alternatively spliced transcript variants have been found for this gene.
Gene ID
10138
Gene Name (eCommerce)
YY1 associated factor 2
Gene Name (Vector)
YY1 associated factor 2
Gene Symbol
YAF2
HGNC ID
HGNC:17363
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
615
Protein Name (eCommerce)
YY1-associated factor 2
RefSeq ID
NM_005748
RefSeq Synonyms
NM_005748, NM_001320080, NM_001190980, NM_001190979, NM_001190977, BC037777,
Research Areas
Complexome,Polycomb Proteins,Transcription Factor/Regulator,Zinc-finger
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
YY1-associated factor 2 (YAF2) binds to MYC and inhibits MYC-mediated transactivation. YAF2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. YAF2 is a component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8IY57].
UniGene ID
Hs.708084
UniProt ID (eCommerce)
Q8IY57

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.