human WIPI2 shRNA silencing Adenovirus

Name 
Ad-h-WIPI2-shRNA
Cat No 
shADV-227794
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human WIPI2.

shADV-227794
Ad-h-WIPI2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human WIPI2 (WD repeat domain, phosphoinositide interacting 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATG18B; Atg21; CGI-50; WD repeat domain phosphoinositide-interacting protein 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2
Description (eCommerce)
WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) is an early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P).WIPI2 may bind PtdIns3P. [taken from www.uniprot.org/uniprot/Q9Y4P8].
Description (Vector)
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 (PubMed 15602573)).(supplied by OMIM, Mar 2008)
Gene ID
26100
Gene Name (eCommerce)
WD repeat domain, phosphoinositide interacting 2
Gene Name (Vector)
WD repeat domain, phosphoinositide interacting 2
Gene Symbol
WIPI2
HGNC ID
HGNC:32225
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1365
ORF Size (bp)
1365 bp
Protein Name (eCommerce)
WD repeat domain phosphoinositide-interacting protein 2
RefSeq ID
NM_015610
RefSeq Synonyms
NM_016003, NM_015610, NM_001278299, NM_001033520, NM_001033519, NM_001033518, BC021200, BC007596, BC004116,
Research Areas
Autophagy
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) is an early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P).WIPI2 may bind PtdIns3P. [taken from www.uniprot.org/uniprot/Q9Y4P8].
UniGene ID
Hs.122363
UniProt ID (eCommerce)
Q9Y4P8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.