human VPS29 shRNA silencing Adenovirus

Name 
Ad-h-VPS29-shRNA
Cat No 
shADV-227524
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human VPS29.

shADV-227524
Ad-h-VPS29-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human VPS29 (VPS29, retromer complex component). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DC15; DC7; PEP11
Description (Vector)
VPS29 belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene.
Gene ID
51699
Gene Name (Vector)
VPS29, retromer complex component
Gene Symbol
VPS29
HGNC ID
HGNC:14340
ORF Size (aa)
561
RefSeq ID
NM_016226
RefSeq Synonyms
NM_057180, NM_016226, NM_001282151, NM_001282150, BC095446, BC032462, BC000880,
Species
human
UniGene ID
Hs.600114

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.