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human USO1 shRNA silencing Adenovirus

Name 
Ad-h-USO1-shRNA
Cat No 
shADV-227280
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human USO1.

shADV-227280
Ad-h-USO1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human USO1 (USO1 vesicle transport factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
general vesicular transport factor p115; P115; Protein USO1 homolog; TAP; transcytosis associated protein; Transcytosis-associated protein; USO1 vesicle docking protein homolog; VDP; vesicle docking protein p115; Vesicle-docking protein
Description (eCommerce)
p115 is a general vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60763].
Description (Vector)
USO1 is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
Gene ID
8615
Gene Name (eCommerce)
USO1 vesicle transport factor
Gene Name (Vector)
USO1 vesicle transport factor
Gene Symbol
USO1
HGNC ID
HGNC:30904
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2886
ORF Size (bp)
2886 bp
Protein Name (eCommerce)
General vesicular transport factor p115
RefSeq ID
NM_003715
RefSeq Synonyms
NM_003715, NM_001290049, BC032654,
Research Areas
Cell Cycle,Golgi Apparatus,Transport
Research Areas (Faceted)
cell_biology,cell_cycle,genetics
Species
human
Target Sentence
p115 is a general vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60763].
UniGene ID
Hs.292689
UniProt ID (eCommerce)
O60763

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.