human UBE4A shRNA silencing Adenovirus

Name 
Ad-h-UBE4A-shRNA
Cat No 
shADV-227052
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UBE4A.

shADV-227052
Ad-h-UBE4A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UBE4A (ubiquitination factor E4A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
E4; RING-type E3 ubiquitin transferase E4 A; ubiquitin conjugation factor E4 A; ubiquitination factor E4A (UFD2 homolog, yeast); UBOX2; UFD2
Description (eCommerce)
Ubiquitin conjugation factor E4 A (UBE4A) binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14139].
Description (Vector)
UBE4A encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene ID
9354
Gene Name (eCommerce)
ubiquitination factor E4A
Gene Name (Vector)
ubiquitination factor E4A
Gene Symbol
UBE4A
HGNC ID
HGNC:12499
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3201
ORF Size (bp)
3201 bp
Protein Name (eCommerce)
Ubiquitin conjugation factor E4 A
RefSeq ID
NM_004788
RefSeq Synonyms
NM_004788, NM_001204077, BC112367, BC111417,
Research Areas
Antigen Processing and Presentation,Immunology,Ubiquitination
Research Areas (Faceted)
cell_biology,immunology
Species
human
Target Sentence
Ubiquitin conjugation factor E4 A (UBE4A) binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14139].
UniGene ID
Hs.602967
UniProt ID (eCommerce)
Q14139

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.