human UBE3A shRNA silencing Adenovirus

Name 
Ad-h-UBE3A-shRNA
Cat No 
shADV-227048
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UBE3A.

shADV-227048
Ad-h-UBE3A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UBE3A (ubiquitin protein ligase E3A). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ANCR; AS; CTCL tumor antigen se37-2; E6-AP; E6AP ubiquitin-protein ligase; EPVE6AP; HECT-type ubiquitin transferase E3A; HPVE6A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; renal carcinoma antigen NY-REN-54; ubiquitin-protein ligase E3A
Description (eCommerce)
E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system.
Description (Vector)
UBE3A encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Gene ID
7337
Gene Name (eCommerce)
ubiquitin protein ligase E3A
Gene Name (Vector)
ubiquitin protein ligase E3A
Gene Symbol
UBE3A
HGNC ID
HGNC:12496
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2619
ORF Size (bp)
2619 bp
Protein Name (eCommerce)
Ubiquitin-protein ligase E3A
RefSeq ID
NM_000462
RefSeq Synonyms
NM_130839, NM_130838, NM_000462, BC009271, BC002582,
Research Areas
Antigen Processing and Presentation,Brain Development,Cancer,Complexome,Host-Virus Interactions,Immunology,Neurobiology,Neurodevelopment,Proteasome,Ubiquitination,Viral Carcinogenesis
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,immunology,infectious_disease,neurobiology
Species
human
Target Sentence
E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system.
UniGene ID
Hs.598862
UniProt ID (eCommerce)
Q05086

Recently Viewed Products

Ad-h-COG4

Cat No: ADV-205669

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.