human TFPI shRNA silencing Adenovirus

Name 
Ad-h-TFPI-shRNA
Cat No 
shADV-225369
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human TFPI.

shADV-225369
Ad-h-TFPI-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TFPI (tissue factor pathway inhibitor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
EPI; LACI; TFI; TFPI1
Description (Vector)
TFPI encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed.
Gene ID
7035
Gene Name (Vector)
tissue factor pathway inhibitor
Gene Symbol
TFPI
HGNC ID
HGNC:11760
ORF Size (aa)
756
RefSeq ID
NM_006287
RefSeq Synonyms
NM_006287, NM_001329241, NM_001329240, NM_001329239, NM_001318941, NM_001032281, BC015514,
Species
human
UniGene ID
Hs.516578

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.