human STIL shRNA silencing Adenovirus

Name 
Ad-h-STIL-shRNA
Cat No 
shADV-224564
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human STIL.

shADV-224564
Ad-h-STIL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human STIL (SCL/TAL1 interrupting locus). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
MCPH7; SCL/TAL1 interrupting locus; SCL-interrupting locus protein; SIL; TAL-1-interrupting locus protein
Description (eCommerce)
SCL-interrupting locus (SIL) plays an important role in embryonic development as well as in cellular growth and proliferation. The long-term silencing of SIL affects cell survival and cell cycle distribution as well as decreases CDC2 activity correlated with reduced phosphorylation of CDC2. SIL is a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15468].
Description (Vector)
STIL encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V-(D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
6491
Gene Name (eCommerce)
STIL, centriolar assembly protein
Gene Name (Vector)
SCL/TAL1 interrupting locus
Gene Symbol
STIL
HGNC ID
HGNC:10879
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3813
ORF Size (bp)
3813 bp
Protein Name (eCommerce)
SCL-interrupting locus protein
RefSeq ID
NM_003035
RefSeq Synonyms
NM_003035, NM_001282939, NM_001282938, NM_001282937, NM_001282936, NM_001048166, BC144089, BC126223,
Research Areas
Apoptosis,Brain Development,Cancer,Cardiology,Cardiovascular,Cell Cycle,Hedgehog Signaling,Neurobiology,Neurodevelopment,Signal Transduction
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction
Species
human
Target Sentence
SCL-interrupting locus (SIL) plays an important role in embryonic development as well as in cellular growth and proliferation. The long-term silencing of SIL affects cell survival and cell cycle distribution as well as decreases CDC2 activity correlated with reduced phosphorylation of CDC2. SIL is a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15468].
UniGene ID
Hs.525198
UniProt ID (eCommerce)
Q15468

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.