human SF3A3 shRNA silencing Adenovirus

Name 
Ad-h-SF3A3-shRNA
Cat No 
shADV-222759
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SF3A3.

shADV-222759
Ad-h-SF3A3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SF3A3 (splicing factor 3a subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
pre-mRNA splicing factor SF3a (60kD); PRP9; PRPF9; SAP 61; SAP61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa
Description (eCommerce)
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
Description (Vector)
SF3A3 encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. SF3A3 has a pseudogene on chromosome 20.
Gene ID
10946
Gene Name (eCommerce)
splicing factor 3a subunit 3
Gene Name (Vector)
splicing factor 3a subunit 3
Gene Symbol
SF3A3
HGNC ID
HGNC:10767
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1506
ORF Size (bp)
1506 bp
Protein Name (eCommerce)
Splicing factor 3A subunit 3
RefSeq ID
NM_006802
RefSeq Synonyms
NM_006802, NM_001320830, BC011523, BC002395,
Research Areas
Gene Expression,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome,Zinc-finger
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
UniGene ID
Hs.77897
UniProt ID (eCommerce)
Q12874

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.