Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human RRP8 shRNA silencing Adenovirus

Name 
Ad-h-RRP8-shRNA
Cat No 
shADV-222017
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RRP8.

shADV-222017
Ad-h-RRP8-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RRP8 (ribosomal RNA processing 8, methyltransferase, homolog (yeast)). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
cerebral protein 1; KIAA0409; NML; nucleomethylin; ribosomal RNA processing 8, methyltransferase, homolog; ribosomal RNA-processing protein 8; RRP8 methyltransferase homolog
Description (eCommerce)
Ribosomal RNA-processing protein (RRP8) is an essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43159].
Gene ID
23378
Gene Name (eCommerce)
ribosomal RNA processing 8
Gene Name (Vector)
ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Gene Symbol
RRP8
HGNC ID
HGNC:29030
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1371
ORF Size (bp)
1371 bp
Protein Name (eCommerce)
Ribosomal RNA-processing protein 8
RefSeq ID
NM_015324
RefSeq Synonyms
NM_015324, BC001071,
Research Areas
Apoptosis,Cell Cycle,Chromatin Remodeling,Epigenetic Regulation of Gene Expression,Immunology,Metabolism/Metabolic Process,Methyltransferase,RNA Processing,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,transcription_translation,immunology,metabolism,genetics
Species
human
Target Sentence
Ribosomal RNA-processing protein (RRP8) is an essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43159].
UniGene ID
Hs.652255
UniProt ID (eCommerce)
O43159

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.