human RPS6KB1 shRNA silencing Adenovirus

Name
Ad-h-RPS6KB1-shRNA

Cat No
shADV-221943

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human RPS6KB1.

shADV-221943

Ad-h-RPS6KB1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RPS6KB1 (ribosomal protein S6 kinase B1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

70 kDa ribosomal protein S6 kinase 1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; p70-S6K 1; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K; S6K1; S6K-beta-1; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; STK14A

Description (eCommerce)

70 kDa ribosomal protein S6 kinase I (p70S6K) encodes a member of the ribosomal S6 kinase family that phosphorylates the 40 S ribosomal subunit protein S6 and eukaryotic translation initiation factor 4B (eIF4B). p70S6 functions in the PI3K and mTOR pathway to induce protein synthesis and cell growth and proliferation in response to growth factors. S6K2 is an isoform of p70S6K.

Description (Vector)

RPS6KB1 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Gene ID

6198

Gene Name (eCommerce)

ribosomal protein S6 kinase B1

Gene Name (Vector)

ribosomal protein S6 kinase B1

Gene Symbol

RPS6KB1

HGNC ID

HGNC:10436

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1356

ORF Size (bp)

1356 bp

Protein Name (eCommerce)

Ribosomal protein S6 kinase beta-1

RefSeq ID

NM_003161

RefSeq Synonyms

NM_003161, NM_001272060, NM_001272044, NM_001272043, NM_001272042, BC053365,

Research Areas

Aging,Angiogenesis,Apoptosis,B-cell Receptor Signaling,BDNF Signaling,Breast Cancer,Cancer,Cardiac Hypertrophy,Cardiology,Cardiovascular,Cell Cycle,EGFR Signaling,EPO Signaling,HIF-1 Signaling,IFN Signaling,IGF-1 Signaling,Immunology,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Leptin Signaling,Leukemia,Mitochondrion,mTOR Signaling,Neurobiology,PI3K/Akt Signaling,Prolactin Signaling,RAC1 Signaling,SCF-KIT Signaling,Signal Transduction,Synapse,T-cell Receptor Signaling,TGF-beta Signaling,TNF Signaling,Translational Control,TSH Signaling,VEGF Signaling

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,neurobiology,signal_transduction,stem_cell_biology,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.463642

UniProt ID (eCommerce)

P23443

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.