human RPS6KA4 shRNA silencing Adenovirus

Name 
Ad-h-RPS6KA4-shRNA
Cat No 
shADV-221938
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RPS6KA4.

shADV-221938
Ad-h-RPS6KA4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RPS6KA4 (ribosomal protein S6 kinase A4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
90 kDa ribosomal protein S6 kinase 4; mitogen- and stress-activated protein kinase 2; MSK2; nuclear mitogen- and stress-activated protein kinase 2; ribosomal protein kinase B; ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RSKB; RSK-B; S6K-alpha-4
Description (eCommerce)
MSK1 is a serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). MSK1 Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. MSK1 also phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75582].
Description (Vector)
RPS6KA4 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene ID
8986
Gene Name (eCommerce)
ribosomal protein S6 kinase A4
Gene Name (Vector)
ribosomal protein S6 kinase A4
Gene Symbol
RPS6KA4
HGNC ID
HGNC:10433
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1575
ORF Size (bp)
1575 bp
Protein Name (eCommerce)
Ribosomal protein S6 kinase alpha-4
RefSeq ID
NM_003942
RefSeq Synonyms
NM_003942, NM_001318361, NM_001300802, NM_001006944, BC047896,
Research Areas
Axon Guidance,Histone Modification,IFN Signaling,Immunology,Insulin Signaling,Interleukin Signaling,Kinase/Phosphatase,MAPK Signaling,Neurobiology,Signal Transduction,TNF Signaling
Research Areas (Faceted)
cell_biology,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
MSK1 is a serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). MSK1 Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. MSK1 also phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75582].
UniGene ID
Hs.105584
UniProt ID (eCommerce)
O75676

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.