human RNF216 shRNA silencing Adenovirus

Name 
Ad-h-RNF216-shRNA
Cat No 
shADV-221411
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RNF216.

shADV-221411
Ad-h-RNF216-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RNF216 (ring finger protein 216). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CAHH; E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; triad domain-containing protein 3; TRIAD3; U7I1; UBCE7IP1; ubiquitin-conjugating enzyme 7-interacting protein 1; ZIN; zinc finger protein inhibiting NF-kappa-B
Description (eCommerce)
Ring finger protein 216 (RNF216) specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains RNF216 are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. RNF216 may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates [taken from NCBI Entrez Gene (Gene ID: 10084)].
Description (Vector)
RNF216 encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
Gene ID
54476
Gene Name (eCommerce)
ring finger protein 216
Gene Name (Vector)
ring finger protein 216
Gene Symbol
RNF216
HGNC ID
HGNC:21698
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2601
ORF Size (bp)
2601 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase RNF216
RefSeq ID
NM_207111
RefSeq Synonyms
NM_207116, NM_207111, BC063825,
Research Areas
Apoptosis,Cancer,Host-Virus Interactions,Response to Virus,Signal Transduction,Toll-like Receptor Signaling,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
Ring finger protein 216 (RNF216) specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains RNF216 are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. RNF216 may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates [taken from NCBI Entrez Gene (Gene ID: 10084)].
UniGene ID
Hs.689456
UniProt ID (eCommerce)
Q9NWF9

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.