Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human RNF213 shRNA silencing Adenovirus

Name 
Ad-h-RNF213-shRNA
Cat No 
shADV-221406
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RNF213.

shADV-221406
Ad-h-RNF213-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RNF213 (ring finger protein 213). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALK lymphoma oligomerization partner on chromosome 17; ALO17; C17orf27; E3 ubiquitin-protein ligase RNF213; KIAA1618; MYMY2; mysterin; MYSTR; NET57; RING finger protein 213; RING-type E3 ubiquitin transferase RNF213
Description (eCommerce)
RNF213 is an E3 ubiquitin-protein ligase involved in angiogenesis. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q63HN8].
Description (Vector)
RNF213 encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. The protein also contains an AAA domain, which is associated with ATPase activity. RNF213 is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. RNF213 is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. Alternative splicing results in multiple transcript variants.
Gene ID
57674
Gene Name (eCommerce)
ring finger protein 213
Gene Name (Vector)
ring finger protein 213
Gene Symbol
RNF213
HGNC ID
HGNC:14539
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
9843
ORF Size (bp)
9843 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase RNF213
RefSeq ID
NM_020914
RefSeq Synonyms
NM_020954, NM_020914, NM_001256071, BC172594,
Research Areas
Cancer,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology
Species
human
Target Sentence
RNF213 is an E3 ubiquitin-protein ligase involved in angiogenesis. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q63HN8].
UniGene ID
Hs.657519
UniProt ID (eCommerce)
Q63HN8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.