human RNASEH2A shRNA silencing Adenovirus

Name 
Ad-h-RNASEH2A-shRNA
Cat No 
shADV-221306
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human RNASEH2A.

shADV-221306
Ad-h-RNASEH2A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RNASEH2A (ribonuclease H2 subunit A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AGS4; aicardi-Goutieres syndrome 4 protein; JUNB; ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease HI large subunit; ribonuclease HI subunit A; RNase H(35); RNase H2 subunit A; RNase HI large subunit; RNASEHI; RNHIA; RNHL; THSD8
Description (eCommerce)
RNASEH2A is the catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. RNASEH2A participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. It also mediates the excision of single ribonucleotides from DNA:RNA duplexes [taken from NCBI Entrez Gene (Gene ID: 10535)].
Description (Vector)
RNASEH2A is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.
Gene ID
10535
Gene Name (eCommerce)
ribonuclease H2 subunit A
Gene Name (Vector)
ribonuclease H2 subunit A
Gene Symbol
RNASEH2A
HGNC ID
HGNC:18518
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
900
Protein Name (eCommerce)
Ribonuclease H2 subunit A
RefSeq ID
NM_006397
RefSeq Synonyms
NM_006397, BC011748,
Research Areas
Cell Cycle,DNA Replication,Replication,RNA Binding,RNA Processing
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
RNASEH2A is the catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. RNASEH2A participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. It also mediates the excision of single ribonucleotides from DNA:RNA duplexes [taken from NCBI Entrez Gene (Gene ID: 10535)].
UniGene ID
Hs.532851
UniProt ID (eCommerce)
O75792

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.