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human RNASE4 shRNA silencing Adenovirus

Name 
Ad-h-RNASE4-shRNA
Cat No 
shADV-221296
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ANG.

shADV-221296
Ad-h-RNASE4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ANG (angiogenin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALS9; HEL168; RAA1; RNASE4; RNASE5
Description (Vector)
ANG is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.
Gene ID
283
Gene Name (Vector)
angiogenin
Gene Symbol
ANG
HGNC ID
HGNC:483
ORF Size (aa)
444
ORF Size (bp)
444 bp
RefSeq ID
NM_001145
RefSeq Synonyms
NM_001145, NM_001097577, BC062698, BC054880, BC020704,
Species
human
UniGene ID
Hs.283749

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.