human RFX5 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human RFX5.
shADV-221073
Ad-h-RFX5-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human RFX5 (regulatory factor X5). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
DNA-binding protein RFX5; regulatory factor X 5; regulatory factor X, 5 (influences HLA class II expression)
Description (eCommerce)
Regulatory factor X-5 (RFX5) is a 75kDa subunit of the regulatory factor X (RFX) heteromeric complex that activates the transcription of major histocompatibility class II (MHC-II) genes by binding MHC-II promoter elements. The RFX complex consists of RFX5, RFXANK, and RFXAP. Mutations in these genes result in a lack of MHCII transcription and lead to the severe combined immunodeficiency disease, Bare Lymphocyte Syndrome (BLS).
Description (Vector)
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.
Gene ID
5993
Gene Name (eCommerce)
regulatory factor X5
Gene Name (Vector)
regulatory factor X5
Gene Symbol
RFX5
HGNC ID
HGNC:9986
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1851
ORF Size (bp)
1851 bp
Protein Name (eCommerce)
DNA-binding protein RFX5
RefSeq ID
NM_000449
RefSeq Synonyms
NM_001025603, NM_000449, BC017471,
Research Areas
Antigen Processing and Presentation,Immunology,Nuclear Receptor Coactivators,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,transcription_translation
Species
human
Target Sentence
Regulatory factor X-5 (RFX5) is a 75kDa subunit of the regulatory factor X (RFX) heteromeric complex that activates the transcription of major histocompatibility class II (MHC-II) genes by binding MHC-II promoter elements. The RFX complex consists of RFX5, RFXANK, and RFXAP. Mutations in these genes result in a lack of MHCII transcription and lead to the severe combined immunodeficiency disease, Bare Lymphocyte Syndrome (BLS).
UniGene ID
Hs.632472
UniProt ID (eCommerce)
P48382
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.