Adenovirus Construction
All viruses made using our Ad-HQ system will express your gene of interest (GOI) with high viral yield and fast delivery.
Why Use Our Adenovirus
Construction Service?
Calibrate Accuracy
Optimize dosages and titers to avoid toxicity or inefficacy.
Enhance Specificity
Confirm target gene effects and minimize off-target results.
Support Quality Control
Validate consistency and safety in AAV production.
Meet Regulatory Standards
Ensure compliance for preclinical and clinical studies.
The Adenovirus Construction Process
We provide custom services for each step of the adenovirus construction process.
Once completed, you will receive a final viral stock of approximately
~1012 viral particles (1×1010-1×1011 PFU)
PROCESS
4-5 weeks to complete
Cloning
Transfection
Adenovirus Packaging
Amplification
CsCI Purification
PFU Titration
Custom plasmids
in 2 weeks
Viral packaging, purification, and QC in 2-4 weeks
Custom plasmids in 2 weeks
VIRAL PACKAGING, PURIFICATION, AND QC IN 2-4 WEEKS
Cloning
Transfection
Adenovirus Packaging
Amplification
Amplification
CsCI Purification
PFU Titration
How to Get Started
For over-expression adenoviruses, provide:
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A plasmid DNA for the specified gene along with its sequence information and a vector map.
If you do not have a plasmid, you can simply send us the sequence or RefSeq# for the GOI.
For shRNA silencing adenoviruses, provide:
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Either a working shRNA in plasmid DNA or the sequence of the shRNA you want to use.
If you don’t have a working shRNA, you can provide us with the name and species (or RefSeq#) for the gene you want to silence, and we will begin with our shRNA screening/validation process to identify a good shRNA for viral production.
For gRNA adenoviruses, provide:
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A plasmid containing your gRNA cassette or the sequence of the gRNA you want to use.
Why Use Vector Biolabs
AccuTiter
AccuTiter ensures accurate and consistent adenovirus titration, eliminating variations and simplifying the traditionally tedious process.
Fast and Seamless Ordering
Experience quick response times and an efficient ordering process for a hassle-free experience.
Tailored Expertise
Receive customized, informed solutions designed to meet your specific research needs.
Reliable Results
Benefit from accurate titers and consistent, stable outcomes in every batch.
Other Adenoviral Services
Alongside our adenoviral construction service, we also provide
Virus amplification
medium (<0.5L of cells) to large scale (1-2 L of culture)
Purification
m2xCsCl purification, titration, and quality control.
If you or a colleague has a virus stock, but aren’t comfortable with the titer, please contact us and we will be happy to assist you.
Adenovirus Amplification