human RAD54B shRNA silencing Adenovirus

Name
Ad-h-RAD54B-shRNA

Cat No
shADV-220651

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human RAD54B.

shADV-220651

Ad-h-RAD54B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RAD54B (RAD54 homolog B (S. cerevisiae)). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

RDH54

Description (Vector)

RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.

Gene ID

25788

Gene Name (Vector)

RAD54 homolog B (S. cerevisiae)

Gene Symbol

RAD54B

HGNC ID

HGNC:17228

ORF Size (aa)

2733

ORF Size (bp)

2733 bp

RefSeq ID

NM_012415

RefSeq Synonyms

NM_012415, NM_001205263, NM_001205262, BC033710, BC001965,

Species

human

UniGene ID

Hs.710768

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.