human PRDM16 shRNA silencing Adenovirus

Name
Ad-h-PRDM16-shRNA

Cat No
shADV-219761

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human PRDM16.

shADV-219761

Ad-h-PRDM16-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRDM16 (PR/SET domain 16). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CMD1LL; KMT8F; LVNC8; MEL1; PFM13

Description (Vector)

The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene ID

63976

Gene Name (Vector)

PR/SET domain 16

Gene Symbol

PRDM16

HGNC ID

HGNC:14000

ORF Size (aa)

3771

ORF Size (bp)

3771 bp

RefSeq ID

NM_022114

RefSeq Synonyms

NM_199454, NM_022114, BC168363, BC161614,

Species

human

UniGene ID

Hs.99500

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.