human PAN2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human PAN2.
shADV-218171
Ad-h-PAN2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human PAN2 (PAN2 poly(A) specific ribonuclease subunit). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
inactive ubiquitin carboxyl-terminal hydrolase 52; PAB1P-dependent poly(A)-nuclease; PAB1P-dependent poly(A)-specific ribonuclease; PAB-dependent poly(A)-specific ribonuclease subunit 2; PAB-dependent poly(A)-specific ribonuclease subunit PAN2; PABP-dependent poly(A) nuclease 2; PAN deadenylation complex catalytic subunit 2; PAN deadenylation complex subunit 2; PAN2 poly(A) specific ribonuclease subunit homolog; PAN2 polyA specific ribonuclease subunit; PAN2-PAN3 deadenylation complex catalytic subunit PAN2; poly(A)-nuclease deadenylation complex subunit 2; ubiquitin specific peptidase 52; ubiquitin specific protease 52; USP52
Description (eCommerce)
PAB-dependent poly(A)-specific ribonuclease subunit PAN2 is the catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q504Q3].
Description (Vector)
PAN2 encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants.
Gene ID
9924
Gene Name (eCommerce)
poly(A) specific ribonuclease subunit PAN2
Gene Name (Vector)
PAN2 poly(A) specific ribonuclease subunit
Gene Symbol
PAN2
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3609
ORF Size (bp)
3609 bp
Protein Name (eCommerce)
PAB-dependent poly(A)-specific ribonuclease subunit PAN2
RefSeq ID
NM_014871
RefSeq Synonyms
NM_014871, NM_001166279, NM_001127460, BC094885, BC024043,
Research Areas
Gene Expression,mRNA Processing,RNA Processing
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
PAB-dependent poly(A)-specific ribonuclease subunit PAN2 is the catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1Â [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q504Q3].
UniGene ID
Hs.273397
UniProt ID (eCommerce)
Q504Q3
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.