human NUF2 shRNA silencing Adenovirus

Name 
Ad-h-NUF2-shRNA
Cat No 
shADV-217224
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NUF2.

shADV-217224
Ad-h-NUF2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NUF2 (NUF2, NDC80 kinetochore complex component). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
cancer/testis antigen 106; CDCA1; cell division cycle associated 1; cell division cycle-associated protein 1; CT106; hNuf2; hNuf2R; hsNuf2; kinetochore protein Nuf2; NUF2, NDC80 kinetochore complex component, homolog; NUF2R
Description (eCommerce)
NDC80 kinetochore complex component (NUF2) acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. NUF2 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BZD4].
Description (Vector)
NUF2 encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
Gene ID
83540
Gene Name (eCommerce)
NDC80 kinetochore complex component NUF2
Gene Name (Vector)
NUF2, NDC80 kinetochore complex component
Gene Symbol
NUF2
HGNC ID
HGNC:14621
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1395
ORF Size (bp)
1395 bp
Protein Name (eCommerce)
Kinetochore protein Nuf2
RefSeq ID
NM_031423
RefSeq Synonyms
NM_145697, NM_031423, BC008489,
Research Areas
Cell Cycle,Cell Division
Research Areas (Faceted)
cell_biology,cell_cycle,genetics
Species
human
Target Sentence
NDC80 kinetochore complex component (NUF2) acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. NUF2 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BZD4].
UniGene ID
Hs.651950
UniProt ID (eCommerce)
Q9BZD4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.